A three-dimensional point spread function for phase retrieval and deconvolution

Deconvolution of vibroacoustic images using a simulation model based on a three dimensional point spread function

Vibro-acoustography (VA) is a medical imaging method based on the difference-frequency generation produced by the mixture of two focused ultrasound beams. VA has been applied to different problems in medical imaging such as imaging bones, microcalcifications in the breast, mass lesions, and calcified arteries. The obtained images may have a resolution of 0.7-0.8mm. Current VA systems based on c...

Enhanced three-dimensional deconvolution microscopy using a measured depth-varying point-spread function.

We present a technique to systematically measure the change in the blurring function of an optical microscope with distance between the source and the coverglass (the depth) and demonstrate its utility in three-dimensional (3D) deconvolution. By controlling the axial positions of the microscope stage and an optically trapped bead independently, we can record the 3D blurring function at differen...

Enhancement of Point Spread Function of Planar Fluorescent Imaging System Using Iterative Deconvolution Method

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One-dimensional iterative algorithm for three-dimensional point-spread function engineering.

We present a new method with which to binarize pupil filters designed to control the three-dimensional irradiance distribution in the focal volume of an optical system. The method is based on a one-dimensional iterative algorithm, which results in efficient use of computation time and in simple, easy to fabricate binary filters. An acceptable degree of resemblance between the point-spread funct...

Algorithm for 3d Localization Microscopy Using Realistic Modeling of the Point Spread Function by Phase Retrieval